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The aim of this study is to research the effect of self-assessment on achievement in an online course at higher education level. The study was conducted with 688 participants (360 male and 328 female) at online Project Culture course at a foundation university in Turkey. A one-way between subjects ANOVA was conducted to compare the effect of self-assessment on achievement depending on attempt number.Our results suggest that when attempts to self-assessment increase achievement level increase as well.