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The current study investigated the effect of different working positions on simulation sickness in 3D Aerodrome Control Training (ACT). Sixteen undergraduate students from a leading school of civil aviation participated in the study. Due to the limited number of enrollment in the institution, a time-series design was implemented. Nausea (e.g., sweating, burping, stomach awareness), disorientation (e.g., dizziness, vertigo, fullness of head) and oculomotor disturbances (e.g. fatigue, headache, eyestrain) were addressed while conducting different tasks in the simulation.