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The interaction between intelligent artificial pedagogical agents and participant prior knowledge on note-taking strategies and learning within a hypermedia learning environment was examined. Sixty college students participated in experimental learning sessions with or without pedagogical agents. Results revealed participants’ note-taking efforts were negatively related to conceptual learning. The presence of agents significantly decreased measurements of quantity and quality of notes but did not foster adaptive note-taking. Notably, this reduction was only observed for low prior knowledge participants, revealing a significant interaction between the adaptive hypermedia environment and learners’ prior knowledge. Scholarly significance of findings is discussed in terms of theoretical and methodological contributions.