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The purpose of this study was to determine the effects of segmentation (learner control) on immediate and delayed knowledge transfer in a multimedia environment. The independent variables of segmentation and non-segmentation, and immediate and delayed assessments were manipulated to assess the effects of segmentation on the participants’ ability to transfer information from a multimedia tutorial. Data was analyzed using a 2X2 factorial design. The results of this study found that segmentation of multimedia tutorials did not result in significant differences in transfer. The results also revealed that the time period between when the tutorials was viewed and when the transfer assessments were taken did significantly affect participants’ ability to transfer information.