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This study tested the effect of the temporal spacing of content retrieval attempts as participants used a web-based retrieval practice tool that scaffolded visual identification of plants and the spelling of their scientific names. Our results demonstrate that in addition to the influence of prior knowledge, the spacing of retrieval practice attempts was a significant predictor of overall learning performance. On the other hand, a simple count of mouse and keyboard interactions with the tool did not predict learning performance. Our findings imply that instructional designers should create opportunities for students to practice retrieving content multiple times during a unit of study, spread over several lessons, rather than providing students with experiences that encourage cramming immediately before testing.