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This qualitative case study investigates the ways in which organizational culture and sub-cultures inform how individuals work across internal boundaries in higher education. Among the results are that individual motivations, awareness and strategies are informed by both the overarching organizational culture and sub-cultural differences. Organizational culture via shared mission and goals motivates individuals to collaborate beyond sub-cultural difference. This motivation requires awareness of others that can be honed to construct strategies for successful collaboration. These strategies – relationship and network building, tailored communication, active listening, advocacy and adaptable approach –provide new motivation and greater awareness of others. Results of this study provide insight into ways individuals can more effectively bridge the loosely-coupled components of their institutions for greater organizational success.