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The purpose of this study is to examine the issues of students’ attitude toward science and its relations with science achievement. Data of Taiwanese fourth and eighth graders from the Trends in International Mathematics and Science Study (TIMSS) 2011 were examined. Quantitative data analyses along with statistical techniques accounting for the properties of international large-scale assessment data were utilized to investigate these issues. The results indicate that students’ affective factors decrease over grades. The relations between the eighth graders’ affective factors and science scores are positively stronger than those of the fourth graders. Meanwhile, students’ self-concept has a greater predictive power than intrinsic interest for science scores in both grades.