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This study investigated the effects of iPad story reading and questioning strategy on preschoolers' vocabulary learning and story comprehension. The participants were randomly assigned to one of four conditions: iPad story, iPad story with questions, paper story, and paper story with questions. Each participant had two reading sessions. At the end of the second reading session, children took a target vocabulary test and comprehension test. The results showed a significant main effect for condition on target vocabulary test. The differential effects of iPad story reading and questioning strategy on preschoolers' literacy learning were discussed.