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This experimental study was intended to examine whether game-based learning (GBL) that encompasses particular game characteristics in the OpenSimulator-supported virtual reality (VR) environment can improve mathematical achievement and motivation for elementary school students toward math learning. In this pre- and post-test experimental comparison study, data were collected from 132 4th graders through an achievement test, and a Short Instructional Materials Motivational Survey (SIMMS). The one-way analysis of covariance (ANCOVA) using a treatment (treatment vs. control) on the achievement indicated a significant effect of game-based learning in VR environment on math knowledge test performance. For motivation, the results indicated that there was no significance difference on the post-test scores on perceived MQLA between the experimental group and the control group.