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The purpose of this pilot study was to explore the use of a Massively Multiplayer Online Game designed around science concepts in high school classrooms. We examined the differences between males and females, and low-level and high-level students in learning gains, enjoyment and gameplay. We found that in our sample, the students’ scores were significantly higher for the posttest than the pretest with a small effect size. In terms of gender, we found that enjoyment scores for females were higher than males although males outperformed in posttest. Lastly, we found that effect size of learning gains for the low-level students was higher than high-level students. Our findings suggest MMOGs have potential to engage girls and low-performing students in science.