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This study examines the role that identity formation plays in encouraging girls to pursue STEM education and careers utilizing data from a cohort-based, informal science enrichment program that targets middle school aged girls. Mixed-methods analyses were employed to examine girls’ attitudes, identities and self-efficacy around STEM. Participants had high initial affinities for science and showed a tentative increase in science identities in the post program condition. Implications for best practice in fostering science engagement and identities in middle school aged girls include the importance of hands-on science activities, the need for enthusiastic relatable role models, and an emphasis on deep understanding of scientific principles.