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Signals (or cues) are added to multimedia learning materials to guide a learners’ attention to critical elements of the materials. This meta-analysis was conducted to examine the effects of text-based cues, picture-based cues, and cues based on eye movements to inform theories of multimedia learning that guide the development of learning materials. Data from 44 independent studies were extracted after searching for experimental studies that met specific inclusion criteria. Overall, the meta-analysis revealed a statistically significant effect size (g = .38) when signaling or cueing is used in multimedia learning environments. This overall effect was moderated by study, presentation and methodological features. The findings have significant implications for educators and instructional designers as well as for multimedia theories.