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This study aims to explore the difference between eHealth literacy rates among college students and senior university students. A mixed method approach was used, including questionnaire surveys. A total of 208 respondents including 65 (31.25%) college students (aged 18–22) and 143 (68.75%) senior university students (aged 55–72) participated in the study. There were significant differences in the overall eHealth literacy and the functional eHealth literacy dimension scores. Most study participants believed that most online health information could be read and understood; however, they were skeptical when evaluating information quality. College students often used websites for solving health problems, while senior university students forwarded health information through communication software.