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To distinguish the construct of interest from intrinsic value, intrinsic motivation, and mastery goal orientation, potential indicators were identified from the existing literature, including cost, competence, and emotion appraisal, that could help differentiate among the four internal motivation constructs. Participants were 200 undergraduates surveyed about their motivations for a STEM course they were currently taking, along with their perceptions of course-related cost, competence, and emotion appraisals. Results indicated that positive and negative emotions moderated the relation between interest and intrinsic value. Further, cost and competence moderated the relation between interest and intrinsic motivation. Finally, competence moderated the relation between interest and mastery goal orientation. Thus, the utility of multiple theories of internal motivation for educational research and practice received support.