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In education, sound is used to communicate information in person and digitally. Most research studies focus on speech sound and little work has been done on non-speech sound. In this study, an interaction-based design is used to design non-speech audio in an interactive online learning environment. An experimental design is conducted in this study to assess the effects of non-speech audio in a customized interactive learning environment. The result shows the use of non-speech audio in an interactive learning environment improves learners’ online learning experience significantly.