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Research on content-related emotions is emerging as a critical area of study and suggests that strong emotions in connection with science teaching can be predictors of the quality of instruction. In the present research, we developed the Science Teacher Emotions Scales (Sci-TES) to measure three emotions considered most relevant in the context of science teaching: enjoyment, anger, and anxiety. The survey was piloted with elementary pre-service teachers (n=185) enrolled in Elementary Science Methods courses. Preliminary data are promising. The scales show good reliabilities and confirmatory factor analysis supports the internal validity of the three-factor model (enjoyment, anger, and anxiety). Additional research needs to be performed with larger sized and more diverse samples in partnership with other elementary teacher preparation programs.