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This study was to investigate the association of attitudes toward cross-gender behavior and social relationship with gay/lesbian peers among middle school students in Taiwan. The sample consisted of 398 male and 415 female students. The results illustrate that female students indicate more positive attitude scores than male counterparts. The predictors account for 26%, 28%, and 16% of social relationship with gay peers in Friendship, Love recognition, and Sympathy of justice, respectively. The strongest predictors are participant gender, Discriminating against cross-gender behavior, and Avoiding associated with cross-gender behavior. The predictors account for 13%, 23%, and 10% of social relationship with lesbian peers in Friendship, Love recognition, and Sympathy of justice, respectively. The strongest predictors are participant gender and Discriminating against cross-gender behavior.