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Studies find relations between negative emotions and higher performance, while others find relations between negative emotions and lower performance. We argue these mixed findings might result from less sophisticated methodologies and few studies examining relations between negative emotional changes, emotion regulation, and performance. To address these challenges, we captured 142 undergraduates’ negative emotions defined by valence and activation guided by Control-Value Theory of Achievement Emotions and Circumplex Model of Emotions across 4 time points with MetaTutor. Latent growth models revealed the intercept of negative activating emotions were related to post-test scores, yet there were no relations between emotion regulation, negative deactivating emotions, and performance. Findings provide theoretical and methodological implications for studying relations between negative emotional changes, emotion regulation, and performance.