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The use of 3D printing in science, technology, engineering, and mathematics (STEM) learning is a promising way for integrated STEM education. This study examined the influence of 3D printing infused STEM integration on students’ STEM career interest, which is essential for students to participate in STEM disciplines and future STEM careers. The participants included 26 teachers across six states in the U.S. and their 1,455 students in primary and secondary classrooms. Cluster analysis and multiple regression analysis indicated that girls were more interested in empathetic STEM careers, whereas boys indicated more interest in analytic STEM careers. While 3D printing integration level was not a significant predictor, STEM integration level positively predicted students’ interest in both analytic and empathetic STEM careers.