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This study examined how high school science teachers’ experience, beliefs, and perspectives regarding applying Self-Regulated Learning (SRL) principles to science lesson plans vary based on their level of SRL knowledge and application skills. Six extreme cases (SRL proficient, emerging) were identified from 20 teachers participating in a Professional Development (PD) program. Teachers’ efficacy for applying SRL and familiarity of SRL were measured before and after the PD and an interview was conducted to understand their experience in incorporating SRL into their lesson plans. Results showed that teacher efficacy and familiarity of SRL increased across all teachers. However, proficient teachers in SRL held a more positive perspective toward the implementation of SRL than emerging teachers. Implications for teacher training are discussed.