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Assessment in online teaching is challenging due to the nature of instructional mode and the assessment complexities. Recognizing such challenges, this study examined the effectiveness of using reflection on selected-response and constructed-response questions as a self-assessment tool in an online learning environment. Students’ perceptions of the usefulness of other online assessment strategies such as asynchronous discussions and hands-on projects were also explored using questionnaires. The results showed that students perceived that a combination of traditional tests and reflection made “an assessment as learning,” but the key was the design of the reflection questions. Potentials of using reflection as a self-assessment tool for improving students’ metacognitive and learning skills in combination with other online assessment strategies were discussed.