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Research suggests that college STEM environments are less likely to support sense of belonging for underrepresented racial/ethnic minority (URM) students and first-generation (FG) college students. In the current study (N = 1,682), we examined five indicators of social and academic belonging in a gateway introductory science course for STEM majors to determine whether these groups experience the same or different belonging concerns. Our results suggested that although URM and FG students’ sense of belonging includes many similarities, there are also unique challenges for each group. URM students reported lower belonging with peers, whereas FG students reported lower academic belonging related to science coursework. Furthermore, our examination of potential explanatory variables suggested that their belonging concerns were explained by different mechanisms.