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This study utilized a mixed method intervention with a sample of early childhood educators (n = 104). They were surveyed on aspects of their relationships with students and responses to child behavior. A subset of survey respondents were randomly assigned to either a professional learning (PL) (n = 20) or control group (n = 22). PL participants participated in a two-day professional learning course that explained child behaviour as a neurophysiological, stress-related phenomenon, as framed by Self-Reg theory. PL participants and controls were re-surveyed eight weeks post-intervention and PL participants were also interviewed. Findings show a significant impact on educator beliefs and practices regarding challenging child behaviour and their understanding of self-regulation as a neurophysiological construct behaviour from program participation.