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Through a qualitative case study approach, this study examines the pedagogical context of training and mentoring students to undertake STEM internships. This paper explores the learning conditions and outcomes of Biotech Partners (BP), a nonprofit organization working at the intersections of bioscience/biotechnology workforce education in collaboration with public high schools and professional experts and organizations in a range of STEM industries. Incorporating multiple stakeholder perspectives (youth, industry partners, mentors, biotech educators), the study unpacks the nature and impacts of these cross-sector partnerships: In what roles and in what ways do bioscience educators and business and industry workforce members motivate students from diverse underrepresented populations to become aware of, interested in, and prepared for careers in the STEM workforce?