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In this study, we examined relationships between teacher self-efficacy and Black female American students’ science achievements. The indicators of teachers’ self-efficacy have been grouped into two categories internal (e.g., knowledge, self-beliefs) and external factors (e.g., school culture, and resources) and those indicators have been analyzed at student-, teacher-, and school-level. The data gathered from PISA 2015 assessment, and results have been analyzed by employing a multilevel approach. The results highlighted that collaboration, teachers’ positive perceptions about school culture and pre-service training positively influenced Black female students’ science achievement, however, their self-efficacy in science content and teaching science content, and availability of resources have not yielded significant contributions to Black female students’ science achievement.