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This multi-case study investigated preservice science teachers’ (PST) self-regulation of teaching. The participants were three PSTs representing low, intermediate, and high self-regulated learners in a certification program for teaching secondary science. Research data came from classroom materials, semi-structured interviews about planning classroom questions, classroom observations, classroom audio recordings, and semi-structured interviews about enacting questions. Findings show that during student teaching, even though the struggles and improving paths of each case were unique, the quality of their self-regulated learning (SRL) practice and teacher questioning all increased. The research elicits PSTs’ SRL practice in the context of planning and enacting classroom questions and suggests a new approach to optimize PSTs’ questioning skills.