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Given the background that findings from previous research studies on gender difference in attitudes toward digital game use were inconsistent. This meta-analysis was conducted to address this issue. From these 41 studies, the cumulative sample size was 35,348, and a total of 133 effect sizes were obtained. Using a three-level random-effects meta-analytic model, a significant effect size of gender difference in attitudes toward digital game use (g = 0.21, 95% CI: [0.10, 0.32]) was found. Moderator analyses showed that study features of game type (entertainment games vs. serious games) and sub-types of attitudes (self-efficacy, affect, and belief) contributed to the heterogeneity of the results across the studies. The implications of the findings, and the limitations and future research considerations were discussed.