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The primary goal of this study was to investigate, in an immersive virtual reality (IVR) environment, the role of learner characteristics in learning and motivational outcomes, including learning retention, perceived learning, cognitive load, self-efficacy, enjoyment, presence, and usefulness. Forty adult participants experienced an IVR nature trail tour involving science learning topics, followed by a knowledge test and multiple perception surveys. MANCOVA (multivariate analysis of covariance) was performed on the data collected, and the results indicated an age by gender interaction effect as well as an age by prior IVR experience interaction effect. Implications of the study will be discussed.