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In this second-order meta-analysis, 14 first-order meta-analyses were included to investigate the effect of multimedia-based education on learning outcomes. In addition, moderator analysis was conducted for instructional setting, discipline, and publication type to understand if they have an impact on learning outcomes. It was found that there was a small positive significant effect size of multimedia-based education on learning outcomes. Through moderator analyses, instructional setting moderated the influence of multimedia-based education on learning whereas disciplines and publication types did not reveal significant moderating effect. Significance and limitations of this second-order meta-analysis are also discussed.