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Existing research shows informal STEM learning experiences such as after-school programs and summer camps present promising avenues for strengthening female students’ STEM self-concept. We seek to examine the moderating effects of female students’ enrollment (co-ed or same-gender STEM camps) on the relationship between female students’ perceptions of mathematics and interest in other STEM subjects. Our findings showed a positive relationship between female students’ perceptions of mathematics and their interest in other STEM subjects. Additionally, our results did not indicate that grade level (middle or high school) or camp type (co-ed or same-gender) influenced female students’ interest in STEM subjects.