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This interdisciplinary research team has examined the students’ reactions to the learning supportive game features based on Basic Psychological Need theory, which is one of the sub-theories of Self-Determination Theory (Ryan & Deci, 2000). These game features were designed to mainly support students’ competence and autonomy need satisfactions. Participants were the 164 undergraduate students enrolled across the four sections of two Biology-related courses in a university in Canada. The participants completed demographic questions before gameplay and a learning-support satisfaction questionnaire and a flow questionnaire after gameplay. Our findings provide empirical data supporting the different use of game features and students’ different perceptions of them.