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This study examines how in-service elementary teachers' life and physical science subject matter knowledge and science teaching efficacy relate to their discipline-specific instructional practices. Findings stem from interviews of teachers who were selected according to preliminary subject matter knowledge and teaching efficacy survey scores. By understanding a relationship between discipline-specific subject matter knowledge, science teaching efficacy, and a teacher's classroom instructional approaches, professional development researchers can better target the support of increasing science teacher knowledge and efficacy to improve classroom science instruction.