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An interesting multiple regression application is the difference-in-difference regression model (DID). The DID model estimates the differential impact of a treatment on subjects in an experimental group versus a control group over time. The DiD regression model includes a key variable, time, in the analysis. The passage of time will change the value of the dependent variable from pre-treatment to post-treatment in a research study. The DiD regression model accounts for the effect of time, thus allowing for interpretation of the treatment effect on the dependent variable over and above the passage of a time effect in the study. A DiD regression model will be demonstrated to illustrate how the equation is written, data analyzed, and interpretations presented.