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Racial and socioeconomic disparities in STEM achievement emerge early and widen over time. Self-perceptions and interest drive achievement, leading to efforts to increase underrepresented students’ interest and efficacy in science. However, a lack of reliable and valid measures to assess elementary students’ scientific self-perceptions and interests hinders our capacity to determine the effectiveness of such efforts. We developed such measures and established their properties in a sample of third-grade students traditionally underrepresented in STEM careers. We present initial evidence for the reliability and validity of these measures and reveal their multi-dimensional nature. These measures can be used by researchers to uncover the nature of young students’ scientific self-perceptions and by practitioners and evaluators to identify effective programs.