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Practical knowledge is an important knowledge base for teachers’ professional development. Developing practical knowledge in pre-service teacher education plays a fundamental role. By collecting teaching videos of 66 student teachers of different disciplines, the coding table was established to capture the practical knowledge of them. It is found that practical knowledge of the student teachers is characterized as stereotyped and fragmented. Reflecting on teacher education curriculum based on empirical data, the study suggests that it is not only necessary to increase the proportion of practice and change teaching methods of teacher education, but also to gradually cultivate the interdisciplinary consciousness of student teachers and guide them to choose concurrent majors, so as to improve the practical orientation of teacher education curriculum.