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Competence beliefs represent a key motivational construct across various motivation theories that are susceptible to change as influenced by various student- and teacher-related characteristics. This study explores the extent to which student- and teacher-related motivational characteristics relate to changes in students’ competence beliefs following 7th grade science teachers who participated in professional learning (PL). Analyses indicated science teachers’ implementation of competence supports taught in the PL and student interest predicted changes in competence beliefs across a chemistry unit. Results highlight the importance of PL focused on instructional practices that support middle school students’ motivation in science classrooms.