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Combining expectancy-value and achievement goal frameworks, we aimed to identify middle school students’ motivational profiles in English and examine the changes or stabilities of different profiles across one semester. Latent profile analysis based on 370 students identified four profiles in both time points: maladaptive, adaptive, average-all, and ambitious-struggling. Latent transition analysis further revealed that about 60% of students stayed in the same profile, among which remaining at the average-all profile demonstrated the highest transition probability. Concerning the general transition tendency, more students were transitioning from more to less favorable profiles, which relatively implicates the declining trend of students’ English motivation. We also examined the covariate effects of gender on profile transition probabilities, but found no significant predicting utility of gender.