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This study tested a moderated mediation model that investigated the empirical link between principal instructional leadership and differentiated instruction, treating teacher professional learning as mediator and teacher autonomy as moderator. Employing a cross-sectional survey design and drawing data from a sizable sample of Turkish teachers, we applied multilevel structural equation modeling (MSEM) with Bayesian estimation to examine the structural associations between the variables under investigation. The preliminary findings corroborated our initial hypothesis, revealing a significant indirect empirical link between instructional leadership and teachers’ reports of the use of differentiated instruction, mediated by teacher professional learning. Currently, we are in the process of running further analyses to test our theoretical model thoroughly.