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This paper explores trauma-informed practice in early childhood education, particularly in transitional kindergarten classrooms. Despite its importance, empirical studies on trauma-informed teaching and teacher education in early childhood education are limited. This study examines how teachers understand and integrate trauma-informed practice using the SAMHSA trauma-informed framework. The participants, five transitional kindergarten educators in California, discussed their experiences and perspectives through online semi-structured interviews. Thematic analysis revealed facilitators and practical and systemic barriers for trauma-informed practices. Understanding these challenges can inform teacher education and support systems to enhance trauma-informed practices in early childhood education, promote resilience and well-being among young children affected by trauma, and address disparities in adverse childhood experiences among diverse student populations.