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As K-12 educators are increasingly being held more accountable to integrate and be responsive to Indigenous histories and perspectives in U.S. classrooms, teacher preparation needs to create opportunities for pre-service teachers to learn to do so through a lens of cultural humility. This mixed methods research study presents findings from a year-long research study examining ways to integrate Indigenous, specifically Dakota and Anishinaabeg, knowledges into an elementary science methods teacher preparation course. We analyze the impact of a collaboration between multiple disciplinary faculty and Indigenous community members to create experiential, place-based learning opportunities for pre-service teachers to develop cultural humility in the context of science education.