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To address declining attitudes towards science in secondary students, the current study investigated an intervention for middle school focused on increasing science interest through hands-on biology research. The current study sought to investigate the development of situational interest (SI) at pre- mid- and post-semester (N = 142), as well as potential gender differences. Results of repeated measures ANOVA showed that time significantly increased Triggered SI and Maintained SI-Feeling, but not Maintained SI-Value. Separating the sample by sex, females showed significantly more Triggered SI and Maintained SI-Feeling than males over time. Females also rated themselves significantly higher in Maintained SI-Value. Implications for research and practice are discussed.