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In today’s educational landscape, exposing students to integrated instruction is crucial yet challenging due to competing priorities and instructional time constraints in K-12 education. Transdisciplinary instruction is one way to face these challenges. This study aims to understand how pre-service teachers (PST) shift their mindsets after participating in a workshop on transdisciplinary instruction design and implementation. This research explores the PSTs’ perceived challenges and anticipated benefits associated with adopting transdisciplinary teaching and how it influences their perspectives on curriculum design and implementation. We meet this purpose by answering the research question: How and to what extent do pre-service teachers' mindsets about content integration as a pedagogical strategy shift after engaging in a workshop that translates theory into practice?