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Teaching students reading comprehension strategies can greatly improve their learning from texts. To support this goal, the iSTART-Early Professional Development (PD) system was designed to expand teachers’ knowledge of the Science of Reading and evidence-based comprehension strategy instruction, through a series of asynchronous instructional videos and learning activities. We report on the efficacy and usability of this PD based on knowledge, self-efficacy, usability, and engagement data from 16 in-service teachers. Overall, teachers found the PD helpful and engaging. They also showed significant improvement in their knowledge of reading comprehension research and self-reported ability to apply this knowledge in the classroom from pre to posttest. These results show initial promise of the iSTART-Early PD system for teachers.