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Emotions can affect healthcare professionals’ performance and decision-making, making it imperative to understand healthcare trainees’ emotion regulation (ER). We investigated which ER strategies healthcare trainees reported during interprofessional simulations and their perceived effectiveness. A pre-post survey study recruited 61 healthcare trainees. Participants reported individual and team ER strategies and their perceived individual ER effectiveness. Individual cognitive change strategies were reported less frequently by trainees than other strategies, with nursing trainees reporting it significantly less frequently than other professions. Trainees’ perceptions of individual ER effectiveness were similar between the professions. These findings provide novel insight into the similarities in ER strategy selection and perceptions between three healthcare training populations, potentially indicating that ER education could be generalizable across different healthcare professions.