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This paper presents a systematic review of pre-service teacher (PST) education for integrated STEM (iSTEM). Forty-nine studies were reviewed using a narrative method, focusing on how PSTs develop conceptualization of iSTEM through iSTEM interventions. The review investigated outcomes of interventions aimed at understanding iSTEM’s integrated nature, approaches to integration, and teaching principles. It also explored various formats and learning activities, including subject-specific methods courses, collaborative subject-specific courses, comprehensive iSTEM courses, workshops, and video analysis. The findings provide insights into designing effective PST interventions to prepare them for iSTEM education in their future classrooms.