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In this study, we explored the design of learning support and its predictive links to preservice teachers’ teaching knowledge and self-efficacy in a virtual reality teaching simulation with AI-powered virtual students. Nineteen preservice teachers were recruited for this cross-sectional quasi-experimental design study. They used learning support throughout the virtual teaching simulation session. We collected data via video-logged duration of learning support use as well as the post-intervention teaching knowledge-skill test and teaching self-efficacy survey. We found a significant predictive link between the duration of learning support use and teaching knowledge-skill test performance, with learning support used in the pretraining and that in the post-simulation stage yielding positive significant impacts. The impacts of learning support were not found on teaching self-efficacy.