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This study examines the relationship between Nature of Science (NOS) understandings and Self-Efficacy (SE) beliefs among preservice elementary teachers (PSETs). It explores the longitudinal growth of NOS and SE beliefs from science content through science methods courses. Using a mixed-methods approach, the research reveals complex relationships between PSETs' evolving NOS understandings and their SE in teaching science through inquiry. Correlation and regression analyses highlight significant relationships, while thematic analysis offers deep insights into the quantitative relationships. The study emphasizes integrating epistemological knowledge with pedagogical skills in science teacher education programs. It aims to improve educational strategies and policies for preparing elementary teachers for their future science teaching.