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This mixed methods study explored two middle school science teachers’ autonomy supportive vs. thwarting responses to instances of student agentic engagement and examined changes in the students’ extrinsic motivation for science over time. Coding of four lessons across three class sections revealed differences between teachers in the proportion and consistency of autonomy-supportive vs. thwarting responses to instances of student agentic engagement. These qualitative patterns aligned with two quantitative results: 1) a difference in post-unit student extrinsic motivation between the teachers and 2) a difference in post- versus pre-unit extrinsic motivation within one class taught by the less autonomy-supportive teacher. Findings highlight the importance of autonomy-supportive teaching practices for promoting student agentic engagement and motivation in service of deeper science learning.