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This study examined the association between gender discrimination and academic achievement among adolescent girls. Gender discrimination referred to the interpersonal bias and discrimination that adolescents’ experience because of their gender. A multidimensional approach was employed comprising several forms (overt and subtle) and sources (peers, school personnel, and adults in the community). Cross-sectional data included 725 adolescent girls aged 13 to 18 years (Mage = 15.90; SDage = 1.23) from two public high schools in California. Hierarchical regression analyses indicated that overt gender discrimination from school personnel was negatively associated with academic achievement. Covariates included age, race/ethnicity, and social class. Findings highlight the need for interventions addressing gender discrimination in educational settings to improve academic outcomes for adolescent girls.