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Early career science teachers (ECST) in high-needs schools face challenges such as insufficient resources and working with diverse students. While several studies have explored these challenges using several lenses, little is known about science-specific challenges that occur in real-time during science teaching. This study uses a qualitative approach to explore real-time challenges that occur during science teaching and how ECSTs respond to these events. Findings reveal that ECSTs real-time instructional challenges were teacher, student or school induced. ECSTs responded by supplementing material resources with personal items and creating problem-solving opportunities to keep the students engaged. The impact on their instruction includes enjoying the outcome of the instructional process while acknowledging the emotionally challenging nature of unexpected occurrences.